Name | Ubiquitin Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
Immunogen |
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to Ubiquitin. |
Gene ID | 7314 |
SwissProt | P62988 |
Synonyms |
40S ribosomal protein S27a; CEP80; Ribosomal protein S27a; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP80; UBIQ; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ubiquitin-CEP80 |
Category | Ubiquitin and Ubiquitin-like proteins |
Background |
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway.Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via ifferent Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the biquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA epair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle egulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; ys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, NA-damage responses as well as in signaling processes leading to activation of the transcription factor NFkappa-B. |
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Ubiquitin Rabbit Polyclonal Antibody
产品编号 | 规格 | 价格 | 货期 | |
UBA-100 | 50 ul | ¥680 | 现货 |
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来源 | 用途 | 交叉反应性 | 分子量 |
Rabbit | WB, ICC, IHC, IF | H, M, R | 10KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry;
FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig;
Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IF | IHC | ICC |
1:500-1:1000 | 1:200 | 1:200 | 1:200 |
保存条件:
Ubiquitin Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推
荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗
体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4ºC保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离
心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳
的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
Western blot analysis of Ubiquitin on different cell lysates
using anti-Ubiquitin antibody at 1/1000 dilution.
Positive control: Lane 1: 293T Lane 2: HepG2 Lane 3: Hela
Lane 4: MCF-2.